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Dr. Maksim Zakhartsev

Experimental & Systems Biology

 Welcome to my profile page. As an experimentalist-biochemist, who came to Systems Biology, I use interdisciplinary approaches to solving challenges in modern biotechnology and adaptation biology. In my research, I do the emphasis on the application of mathematical modeling for prediction and better understanding or optimization of bioprocesses at the system level.

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Competence

  • Biochemistry
  • Analytical biochemistry
  • Systems Biology
  • Mathematical modeling of biosystems
  • Analysis and optimization of bioprocesses
  • Biotechnology
  • Metabolic adaptation
  • Teaching

Academic Degree

Ph.D. in Biochemistry (2003) fom University of Antwerp (Belgium)

Professional networks

ResearchGate

LinkedIn

XING

Experience in Conceptual platforms

Biochemistry (1991+)

Analytical biochemistry (1991+)

Bioinformatics (2004+)

Quantitative biology (1998+)

Metabolic engineering (2005+)
Biosystems engineering (2007+)
Biochemical engineering (2003+)

Systems biology (2007+)

Expedition experience

field scientist

diver

Application profile

Location:

Wet (50%) and Dry (50%) lab

Scientific research in:

biotechnology, metabolic engineering, systems biology, experimental biology, HTS
process and workflow development and their optimization

Research interests:

metabolic adaptation of ectotherms to temperature and other hard environmental factors with further purpose of use the knowledge in biotechnological applications

Scientific work:

research and experiment planning, build experimental setup, data acquisition, data analysis, mathematical modelling, result interpretation
reporting: SOP, process documentation, reports, presentations, talks, conferences, lecturing, research papers

Workspace:

individual work, team work, team leading, project management

Research objects

yeasts, bacteria

humal cells

marine moluscs, fishes

plants

Modeling and Data analysis

Network reconstruction

Stoichiometric modeling (incl. FBA, MFA)

ODE-based mathematical modeling of dynamic material balance of biological processes

Model parametarization

Statistical analysis

Bioinformatics

Biothermodynamics

Regression analysis

Modeling, Simulation & Analyzing Tools

MATLAB

BioUML

COPASI

CellDesigner

InSilico Discovery

R

Statistica

GraphPad Prism

SigmaPlot

Origin

Analytical & Experimental methods

Chromatography, mass-spectrometry

Metabolomics, proteomics, transcriptomics

Experimental biology

Fermentation techniques

Enzyme kinetics

HTS methods

Data management

FileMaker

FAIRDOM

Education

Biochemistry (Ph.D.)

University of Antwerp, Belgium 1998 - 2003

Comparative Biochemistry (Graduate program)

Institute of Marine Biology, Russian Academy of Sciences (RAS), Vladivostok, Russia 1995 - 1998

Biochemistry (Diploma)

Far Eastern Federal University (FEFU), Vladivostok, Russia 1987 - 1992

Extension courses

Q-Exactive biotech operations

ThermoFisher Scientific, University of Hohenheim, Stuttgart, Germany09/2014

Computational Modeling in Biological Network

Institut Curie, Paris, France09/2013

Data driven computational modeling of cellular signaling pathways

Max-Planck-Institute of Biochemistry (Matthias Mann), München, Germany06/2013

Fundamentals of Pharmacology

through Coursera, Perelman School of Medicine, University of Pennsylvania, USA09/2012

Data Management for Systems Biology II

Universidad de Verano de Adeje, Tenerife, Spain07/2009

3rd FEBS Advanced Lecture Course on Systems Biology.

Alpbah course (Hans Westrhoff), Austria03/2009

Biosystems Engineering: Bioreactors and cell factories

Braunwald course (Matthias Reuss), Switzerland09/2007

Metabolic Engineering and Systems Biology

Danish Technical University (Jens Nielsen), Denmark06/2006

DigiSal Centre for Integrative Genetics, Norwegian University of Life Sciences, Ås, Norway, 01/2017 - 12/2018

Position: Researcher (post-doc), project leader Dr.Jon Olav Vik

Reconstruction of whole-genome metabolic model of Atlantic salmon Salmo salar. The goal of the project is optimisation of the fish growth by means of diet for application in salmon farming • network reconstruction • Flux Balance Analysis • integration of high-throughput experimental data • model parameterization. The model aims to optimize a whole range of possible diets of farmed salmon. Gut microbiota will receive particular attention.

Object: salmon Salmo salar

Research area & Technologies:

  • multi-compartmental metabolic model
  • growth optimization
  • modeling (InSilico Discovery)

Extremopharm Plant Systems Biology, University of Hohenheim, Stuttgart, Germany, 01/2016 - 12/2017

Position: Researcher (post-doc), project leader Prof.Elizaveta Bonch-Osmolovsraya

Search of novel antibiotics in the communities of thermophilic microorganisms

Object: community of thermophilic microorganisms

Research area & Technologies:

  • MS-based shotgun proteomics (nLC-MS/MS)
  • community proteomics
  • modeling (InSilico Discovery)
  • community Flux Balance Analysis

ZucAT Plant Systems Biology, University of Hohenheim, Stuttgart, Germany, 01/2014 - 12/2015

Position: Researcher (post-doc) in group of Prof.Waltraud Schulze

Optimization of large-scale proteomics using Orbitrap MS analyser Q Exactive Plus (ThermoScientific) • development of milti-compartment stoichiometric model of central carbon and energy metabolisms of growing Arabidopsis thalianain relation to sucrose transportation • optimization of phosphopeptide enrichment method • Arabidopsiskinome: exploration of possibilities for modeling of plant signaling pathways • dynamic interactome of the H+-ATPase: phosphoproteomics / PPI • SCX and RP HPLC of peptides • UltraHPLC (Easy nLC, ThermoScientific) • sample preparation • data analysis in MaxQuant • peptide library construction

Object: cress Arabidopsis thaliana

Research area & Technologies:

  • plant systems biology
  • MS-based shotgun proteomics (nLC-MS/MS)
  • sample preparation
  • modeling (MATLAB, InSilico Discovery)
  • multi-compartmental metabolic model
  • data mangement (FAIRDOM)

Teaching:

  • practical course on proteomics

>10K Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany, 01/2013 - 05/2013

Position: Researcher (post-doc) in group of Prof.Mathias Mann

Identification of >10k proteins in a single analysis • MS centric aproach in shotgun proteomics using LC-MS/MS: deep proteomics • Orbitrap MS analyser Q Exactive (ThermoScientific) • SCX and RP HPLC of peptides • UltraHPLC (Easy nLC, ThermoScientific) • sample preparation • data analysis in MaxQuant • peptide library construction

Object: HeLa cell line

Research area & Technologies:

  • human cell systems biology
  • MS-based shotgun proteomics (nLC-MS/MS)
  • chromatoraphy
  • sample preparation
  • bioinformatics
  • data analysis + modeling (MATLAB)

Yeast energy metabolism Center Systems Biology, University of Stuttgart, Germany, 01/2012 - 12/2012

Position: Freelancer in group of Prof.Mathias Reuss

Modeling dynamic response of yeast central carbon and energy metabolism using data obtained in SysMO: MOSES project.

Object: yeast Saccharomyces cerevisiae

Research area & Technologies:
  • data analysis • modeling (MATLAB, InSilico Discovery)
  • data management (FAIRDOM)

MedSys: Drug-iPS Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Germany, 03/2010 - 12/2011

Position: Researcher (post-doc) in group of Prof.Stefan Wölfl

MedSys project “Drug based induction of pluripotent human stem cells derived from human somatic cells”.

Object: human fibroblasts

Research area & Technologies:

  • medical systems biology
  • drug discovery
  • screening assay development
  • target identification
  • data analysis • modeling (MATLAB, InSilico Discovery)

Teaching:

  • metabolic engineering
  • practical courses on biochemistry and bioanalytics
  • seminars

SysMO: MOSES Institue of Biochemical Engineering, University of Stuttgart, Germany, 07/2007 - 02/2010

Position: Researcher (post-doc) in group of Prof.Matthias Reuss and in collaboration with Prof.Hans Westerhoff

SysMO project “Microorganism Systems Biology: energy and Saccharomyces cerevisiae(MOSES)”: dynamic modeling of central carbon and energy metabolism of yeast. The glucose pulse was used to perturb steady state continuous yeast culture, the transient concentrations of metabolites were measured and reconstructed trajectories used for parameter estimation of the dynamic model. Rapid sampling, quenching, extraction and analysis techniques were used to measure transient concentrations of both intra- and extra-cellular metabolites. The obtained quantitative information is integrated into the metabolic flux distributions. The main focus of the subsequent kinetic analysis are enzymatic reactions in the central metabolism which are metabolically regulated via dynamic changes in substrates and cometabolites, such as AMP, ADP and ATP. The dynamic model links dynamics of key metabolite (e.g. cAMP, ATP, AICAR, etc) in the response to cellular stress in yeast with transcriptional gene regulation (e.g. SNF1, PHO1, etc). The integrated model aims at a dynamic description of the regulation of the energy metabolism of the yeast cell induced by the substrate perturbations.

Object: yeast Saccharomyces cerevisiae

Research area & Technologies:
  • yeast systems biology
  • cellular energy metabolism
  • experimental biology • perturbation experiments
  • fermentations • process automation
  • chromatoraphy (GC-MS, HPLC) • sample preparation
  • dynamic metabolomics • robotized metabolomics
  • network reconstruction
  • stoichiometric modeling, FBA, MFA (InSilico Discovery)
  • dynamic modeling & model parameterization (MATLAB)
  • data mangement (FAIRDOM)

Yeast energy metabolism Marine Animal Ecophysiology, Alfred Wegener Institute, Bremerhaven, Germany, 07/2006 - 06/2007

Position: Visiting researcher (post-doc) in group of Prof.Hans Otto Pörtner

Project initiative “Metabolic compensation of temperature effect in yeast central and energy metabolism”: yeast in vivoATP balance in respect to growth rate and temperature: (i) ATP yielding coefficent for biomass synthesis (YxATP) and (ii) rate of ATP consumption for maintenance (mATP). The rate of ATP synthesis is measured as a steady state flux from Pi to ATP by in vivo 31P NMR saturation transfer method using double resonance technique.

Object: yeast Saccharomyces cerevisiae

Research area & Technologies:
  • yeast systems biology
  • metabolic adaptation
  • cellular energy metabolism
  • fermentations
  • NMR based metabolomics
  • modeling (MATLAB)

Biochemical Engineering Biochemical Engineering, Jacobs University, Bremen, Germany, 06/2003 - 05/2006

Position: Docent in group of Prof.Ulrich Schwaneberg

Participation in BioFuelCell project: directed protein evolution of glucose oxidase from Aspergilus nigerfor the human in-body application in implantable bio-fuel cell. Conductiong of independent research: yeast metabolic engineering • biothermodynamics • whole genome microarray analysis • mass spectrometry

Object: yeast Saccharomyces cerevisiae

Research area & Technologies:

  • yeast metabolic engineering
  • fermentations
  • bioanalytics • enzyme kinetics
  • bioinformatics
  • chromatography (FPLC) • protein purification
  • downstream processing
  • HTS assay development • high throughput platform technologies/devices
  • statistics

Teaching:

  • biochemistry
  • biochemical engineering
  • metabolic engineering
  • high throughput screening technologies
  • practical courses
  • seminars

CLICOFI Laboratory of Biology, Ecophysiology, Biochemistry and Toxicology, University of Antwerp, Belgium, 12/1998 - 05/2003

Position: Pd.D. student in group of Prof.Ronny Blust

CLICOFI project "Effects of climate induced temperature change on marine coastal fishes". Research of physiological and biochemical mechanisms of marine fish adaptation to seasonal temperature variation: thermal physiology • rate of oxygen consumption • analysis of enzyme kinetics and thermodynamics, with purpose to predict a possible impact of the global ocean warming on fish stock. Collaborative experimental work with research groups in AWI (Bremerhaven, Germany) and University of Bergen (Norway)

Object: eelpout Zoarces vivparus , cod Gadus morhua

Research area:

  • experimental biology
  • fish physiology
  • biochemical adaptation
  • analytical biochemistry
  • enzyme kinetics
  • ELISA • Western Blot
  • enzyme kinetics and thermodynamics
  • high throughput platform devices

COOM Laboratory of Gas Chromatography, Department of Biochemistry, Far Eastern State University, Vladivostok, Russia, 09/1990 - 05/1992

Position: research-assistant in spin-off company Dr.Nikolay Naumenko

Research: development and manufacturing of capillary columns and accessories for gas-liquid chromatography • instrument maintenance and assembly • GC methods development

Object: mollusc chiton Ponerplax costata

Research area:

  • complete cycle of capillary column manufacturing, testing, on-site delivery, mounting, including instruments rebuilding to adapt capillary columns and maintenance
  • manufacturing of fatty acids methyl esters (FAME) standards for calibration of GC analysis
  • development and installation the GC methods (alcohols, drugs, pesticides, fuels, pharmaceutic agents, fatty acids and etc) for customers (academics, forensic medicine, food control agencies, environmental protection agencies, customhouses and etc)

Subjects

  • Advanced Biochemical Engineering Laboratory Course
  • Advanced Biochemistry and Molecular Biology
  • Basic biotechnology (full academic course)
  • Bioanalytical practical courses
  • Biocatalysis and Biocatalytic Technologies (full academic course)
  • Biological Recognition
  • Experimental Systems Biology
  • Food Biotechnology (full academic course)
  • Field trips
  • General Biochemical Engineering (full academic course)
  • High Throughput Screening Technology (full academic course)
  • Metabolic Engineering & Systems Biology (full academic course)
  • Literature club
  • Seminars and Colloquiums
  • Students’ supervision in guided research

Institutions

  • Far Eastern Federal University (Vladivostok, Russia)
  • Institute of Cytology and Genetics (Novosibirsk, Russia)
  • Jacobs University (Bremen, Germany)
  • University of Heidelberg (Germany)
  • University of Hohenheim (Stuttgart, Germany)
  • Ural Federal University (Ekaterinburg, Russia)

PERSONAL DATA

Birthday 04 October 1970

Family status married, two children (2002,2003)

Citizenship Russian Federation

 + German permanent residence permit

 + Norwegian residence permit

Address Stuttgart, Germany

Languages

Russian native
English fluent
Deutsch B2-C1

Contact Me

Cell +49 170 992 7633

Email maksim.zakhartsev@googlemail.com

Website www.zakhartsev.info

Hobbies

free/-diving

swimming, cycling, running

extreme hiking

photo

In progress

  1. Zakhartsev, M., Lapin, A. and Reuss, M. Dynamic model of yeast Saccharomyces cerevisiae nucleotide salwage pathway. The research/modeling article is in preparation for FEBS Journal.
  2. Zakhartsev, M. Grey-box model on the temperature dependent growth rate of yeast Saccharomyces cerevisiae. The research/modeling article is in preparation for FEBS Journal.
  3. Zakhartsev, M., Sokolova, T., Gavrilov, S. Underwater collection of thermophilic microorganisms in the hot springs in shallow marine. The research article is in preparation for JoVE: Journal of Visualized Experiments.

Journals & Books

  1. Zakhartsev, M., Ruess, M. Cell size and morphological properties of yeast Saccharomyces cerevisiae in relation to growth temperature. The research article has been submited to FEMS Yeast Research.
  2. Chelomin, V., Slobodskova, V., Zakhartsev, M., Kukla, S. (2017) Genotoxic potential of copper oxide nanoparticles in the bivalve mollusk Mytilus trossulus. Journal of Ocean University of China (Oceanic and Coastal Sea Research), 16(2): p.339-345. DOI:10.1007/s11802-017-3133-y
  3. Zakhartsev, M., Medvedeva, I., Orlov, Y., Akberdin, I., and Schulze, W., (2016) Metabolic model of central carbon and energy metabolisms of growing Arabidopsis thaliana in relation to sucrose translocation. BMC Plant Biology, 16(1): p.262. DOI: 10.1186/s12870-016-0868-3 The model and support files are available for download under DOI: 10.15490/seek.1.investigation.74.9
  4. Zakhartsev, M., Pertl-Obermeyer, H., and Schulze, W. (2016) Chapter 18: From (phospho)proteome to modelling of plant signalling pathways. In Proteomics in Systems Biology, Editor: Reinders, J. Springer New York, Series volume 1394: p. 245-259. DOI: 10.1007/978-1-4939-3341-9_18
  5. Belskaya, N.P., Lugovik, K.I., Bakulev, V.A., Bauer, J., Kitanovic, I., Holenya, P., Zakhartsev, M., and Wölfl, S. (2016) The new facile and straightforward method for synthesis of 4H-1,2,3-thiadiazolo[5,4-b]indoles and their antiproliferative activity. European Journal of Medicinal Chemistry, 108: p. 245-257. DOI: 10.1016/j.ejmech.2015.11.011
  6. Zakhartsev, M., Yang, X., Pörtner, H.O., and Reuss, M. (2015) Metabolic efficiency in yeast Saccharomyces cerevisiae in relation to temperature dependent growth and biomass yield. Journal of Thermal Biology, 52: p. 117-129. DOI: 10.1016/j.jtherbio.2015.05.008
  7. Zakhartsev, M., Vielhauer, O., Horn, T., Yang, X., and Reuss, M. (2015) Fast sampling for quantitative microbial metabolomics: New aspects on cold methanol quenching - metabolite co-precipitation. Metabolomics, 11(2): p. 286-301. DOI: 10.1007/s11306-014-0700-8
  8. Verma, M., Zakhartsev, M., Reuss, M., and Westerhoff, H. (2013) 'Domino' systems biology and the 'A' of ATP. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1827(1): p. 19-29. DOI: 10.1016/j.bbabio.2012.09.014
  9. Vielhauer, O., Zakhartsev, M., Horn, T., Takors, R., and Reuss, M. (2011) Simplified absolute metabolite quantification by GC-IDMS on the basis of commercially available source material. Journal of Chromatography B: Biomedical Applications, 879(32): p. 3859-3870. DOI: 10.1016/j.jchromb.2011.10.036
  10. Belcheva, N., Zakhartsev, M., Dovzhenko, N., Zhukovskaya, A., Kavun, V., and Chelomin, V. (2011) Anthropogenic pollution stimulates oxidative stress in soft tissues of mussel Crenomytilus grayanus (Dunker1853). Ocean Science Journal, 46(2): p. 85-94. DOI: 10.1007/s12601-011-0008-9
  11. Zakhartsev, M. and Bock, C. (2010) Miniaturized device for agitating a high density yeast suspensions which is suitable for in vivo NMR applications. Analytical Biochemistry, 397(2): p. 244-246. DOI: 10.1016/j.ab.2009.10.011
  12. Zakhartsev, M., Momeu, C., and Ganeva, V. (2007) High throughput liberation of water soluble yeast content by irreversible electropermeation (HT-irEP). Journal of Biomolecular Screening, 12(2): p. 267-275. DOI: 10.1177/1087057106296910
  13. Zakhartsev, M. and Momeu, C. (2007) Purification of glucose oxidase from complex fermentation medium using tandem chromatography. Journal of Chromatography B: Biomedical Applications, 858(1-2): p. 151-158. DOI: 10.1016/j.jchromb.2007.08.015
  14. Zakhartsev, M., Lucassen, M., Kulishova, L., Deigweiher, K., Smirnova, Y., Zinov'eva, R., Mugue, N., Baklushinskaya, I., Pörtner, H.O., and Ozernyuk, N. (2007) Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis: functional consequences for the enzyme. FEBS journal, 274(6): p. 1503-1513. DOI: 10.1111/j.1742-4658.2007.05692.x
  15. Ruoff, P., Zakhartsev, M., and Westerhoff, H. (2007) Temperature compensation through systems biology. FEBS journal, 274(4): p. 940-950. DOI: 10.1111/j.1742-4658.2007.05641.x
  16. Zhu, Z., Momeu, C., Zakhartsev, M., and Schwaneberg, U. (2006) Making glucose oxidase fit for biofuel cell applications by directed protein evolution. Biosensors & Bioelectronics, 21(11): p. 2046-2051. DOI: 10.1016/j.bios.2005.11.018
  17. Belcheva, N., Zakhartsev, M., Silina, A.V., Slinko, E., and Chelomin, V. (2006) Relationship between shell weight and cadmium content in whole digestive gland of the Japanese scallop Patinopecten yessoensis (Jay). Marine Environmental Research, 61(4): p. 396-409. DOI: 10.1016/j.marenvres.2005.12.001
  18. Zakhartsev, M., de Wachter, B., Johansen, T., Pörtner, H.O., and Blust, R. (2005) Hsp70 is not a sensitive indicator of thermal limitation in the North Atlantic cod (Gadus morhua). Journal of Fish Biology, 67(3): p. 767-778. DOI: 10.1111/j.0022-1112.2005.00778.x
  19. Chelomin, V., Zakhartsev, M., Kurilenko, A., and Belcheva, N. (2005) An in vitro study of the effect of reactive oxygen species on subcellular distribution of deposited cadmium in digestive gland of mussel Crenomytilus grayanus. Aquatic Toxicology, 73(2): p. 181-189. DOI: 10.1016/j.aquatox.2005.03.009
  20. Zakhartsev, M., Pörtner, H.O., and Blust, R. (2004) Environmentally low-temperature kinetic and thermodynamic study of lactate dehydrogenase from Atlantic cod (G. morhua) using a 96-well microplate technique. Analytical Biochemistry, 330(1): p. 10-20. DOI: 10.1016/j.ab.2004.03.070
  21. Zakhartsev, M., Johansen, T., Pörtner, H.O., and Blust, R. (2004) Effects of temperature acclimation on lactate dehydrogenase of cod (Gadus morhua): genetic, kinetic and thermodynamic aspects. Journal of Experimental Biology, 207(1): p. 95-112. DOI: 10.1242/jeb.00708
  22. Zakhartsev, M., de Wachter, B., Sartoris, F.J., Pörtner, H.O., and Blust, R. (2003) Thermal physiology of the common eelpout (Zoarces viviparus). Journal of Comparative Physiology Part B, 173(5): p. 365-378. DOI: 10.1007/s00360-003-0342-z
  23. Kurilenko, A., Zakhartsev, M., and Chelomin, V. (2002) In vivo effect of copper ions on transbilayer distribution of aminophospholipids in synaptosomal membrane of walleye pollock (Theragra chalcogramma). Aquatic Toxicology, 58(3-4): p. 131-136. DOI: 10.1016/S0166-445X(01)00224-7
  24. Pörtner, H.O., Berdal, B., Blust, R., Brix, O., Colozimo, A., De Wachter, B., Giuliani, A., Johansen, T., Fischer, T., Knust, R., Lanning, G., Naevdal, G., Nedenes, A., Nyhammer, G., Sartoris, F.J., Serendero, I., Sirabella, P., Thorkildsen, S., and Zakhartsev, M. (2001) Climate induced temperature effects on growh performance, fecundity and recruitment in marine fish: developing a hypothesis for cause and effect relationship in Atlantic cod (Gadus morhua) and common eelpout (Zoarces viviparus). Continental Shelf Research, 21(18-19): p. 1975-1997. DOI: 10.1016/S0278-4343(01)00038-3
  25. Zakhartsev, M., Chelomin, V., and Belcheva, N. (2000) The adaptation of mussels Crenomytilus grayanus to cadmium accumulation result in alterations in organization of microsomal enzyme-membrane complex (non-specific phosphatase). Aquatic Toxicology, 50(1-2): p. 39-49. DOI: 10.1016/S0166-445X(99)00097-1
  26. Zakhartsev, M., Naumenko, N., and Chelomin, V. (1998) Nonmethylene-interrupted fatty acids in membrane phospholipids of the Crenomytilus grayanus. Russian Journal of Marine Biology, 24(3): p. 183-186.
  27. Chelomin, V., Belcheva, N., and Zakhartsev, M. (1998) Biochemical mechanisms of adaptation to cadmium and copper ions in the mussel Mytilus trossulus. Russian Journal of Marine Biology, 24(5): p. 330-336.

Patents

  1. Zakhartsev, M. and Bock, C. (2009) Agitating device for high density cell suspensions suitable for perturbation-sensitive monitoring methods [Einrichtung zum Rìhren von Fluiden mittels Gasblasen]. The patent application DE 10 2009 013 930.3 was filed on 22.03.2009. D.P.-u. Markenamt (Germany).
  2. Zakhartsev, M. and Ganeva, V. (2006) High throughput electropermeation system. The patent application EP06110993.0 was filed on 10.03.2006. E.P. Office (Germany).
  3. Zakhartsev, M., Blust, R., and Verhaert, K. (2004) Autonomous device with active temperature regulation. The patent application WO2004EP02705 20040316 was filed on 23.03.2004. The patent WO2005097325(A1) was published on 20.10.2005. E.P. Office (Belgium).
  4. Zakhartsev, M. and Blust, R. (2001) Temperature-controlled device and method suitable for spectroscopic analysis. The patent application EP20010870130 20010613 was filed on 14.12.2001. The patent EP1266691(A1) was published on 18.12.2002. E.P. Office (Belgium).

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